Caulfield Cup winner Fawkner will not back up in the $3 million Cox Plate at Moonee Valley on Saturday.
Owner Lloyd Williams, who had six horses among the third acceptances, has opted for three to run in the Group One 2040m race - defending Melbourne Cup winner Green Moon, Seville and Masked Marvel.
Along with Fawkner, Herbert Power Stakes winner Sea Moon and Sydney Cup winner Mourayan are also out.
Sea Moon and Mourayan have been nominated for Saturday's Group Two Moonee Valley Cup (2500m).
Green Moon, to be ridden by Brett Prebble, finished a luckless seventh as favourite in last year's Cox Plate.
Hugh Bowman keeps the ride on Seville after partnering the six-year-old to a last-start triumph in the Group One Metropolitan (2400m) in Sydney.
Caulfield Cup winning jockey Nick Hall has been booked to ride Masked Marvel, but that will depend whether he is successful in an appeal on Tuesday against a 10-meeting suspension for careless riding.
Both Sea Moon and Mourayan have been nominated for Saturday's $273,000 Group 2 Drake International Cup (2500m) while Fawkner, who has received a 1.5kg penalty Melbourne Cup penalty, is unlikely to run again until the Melbourne Cup.