Australian centre Kim Green will miss the opening Test of netball's Quad Series because of a foot injury.
The 44-Test stalwart won't play in Australia's fixture against England in Adelaide on Sunday, with Caitlin Thwaites set to make her Test debut.
The Diamonds' clash with England will be followed by New Zealand meeting South Africa in a Test match double-header at Adelaide's Entertainment Centre.
Australian coach Lisa Alexander says Green's injury with an ongoing foot complaint is a blow.
"We are certainly very hopeful she will take further part in the series," Alexander told AAP on Saturday.
"She is one of our greatest centres that we have got in the country and we are very keen to have her out there and playing."
Goal shooter Thwaites was included in Australia's dozen-strong squad for the first of six Tests in the inaugural Quad Series.
The four nations will play three Tests in Australia, and then three in New Zealand in a series ending on November 1.
"It's really exciting," Alexander said of the format.
"Definitely from a coaching perspective, preparing for three different opponents is challenging but it's also really interesting.
"And being able to have the ability to rotate the squad will be of enormous benefit for us and also for the individual development of the players.
"We're very conscious of the fact we want to be able to have the ability to change our combinations and for that to happen seamlessly."
Alexander said the heavy workload of the series would ensure opportunity for all of her squad.
"We'll be testing ourselves out as a team continually," she said.
"It is quite a big load, six Tests, and all the travel that is involved as well, so it's really important that we use the great players that we have - the 14 players that we have got in our Diamonds team all deserve their positions in the team.
"You need to be able to put those players out on court and show the confidence that we do have in those players to perform for Australia."