The Matildas will be relying on two rookie teenage goalkeepers to keep Olympic gold medallists United States at bay during their upcoming tour in America.

The 20-player squad is missing senior goalkeepers Lydia Williams (knee), Casey Dumont (groin) and Melissa Barbieri (pregnancy), and instead will hinge on two rookie teenage goalkeepers - 17-year-old Brianna Davey from Melbourne Victory and 18-year-old Canberra United keeper Mackenzie Arnold.

The Australian side will take on the world No.1 US in two matches in Los Angeles and Denver on September 16 and 19 respectively, after a warm-up game against Haiti in Indianapolis on September 13.

"Two matches against the world's top team on their home soil is a daunting challenge to say the least, but it's the sort of test that will help us in the long-term," coach Tom Sermanni said.

FIFA Women's World Cup All-Star fullback Elise Kellond-Knight is continuing knee rehabilitation and will also be absent, but attacking midfielder Samantha Kerr will rejoin the squad after a year-long absence.

Kerr was a key member of the Matildas' 2010 Women's Asian Cup-winning team and the 2011 FIFA World Cup campaign, but has missed a lot of game time due to a knee reconstruction.

"These games are part of celebration tour by the USA after winning gold in London, so our job is to spoil the party if we can," Sermanni said.

The squad departs for North America on Monday 10 September.

Matildas squad for US Tour: Teigen Allen, Laura Alleway, Mackenzie Arnold, Danielle Brogan, Ashley Brown, Stephanie Catley, Brianna Davey, Lisa de Vanna, Caitlin Foord, Emily Gielnik, Kathryn Gill, Michelle Heyman, Alanna Kennedy, Samantha Kerr, Collette McCallum, Clare Polkinghorne, Kyah Simon, Servet Uzunlar, Emily van Egmond, Sarah Walsh.