A stunning Jermaine Defoe strike 10 minutes from time handed England their first win against Italy in 15 years, the Tottenham hitman sealing a 2-1 victory for Roy Hodgson's men.
England had to come from behind, with Daniele De Rossi heading in to give the Euro 2012 runners-up a 15th-minute lead before Phil Jagielka equalised 12 minutes later with another header.
But just when it appeared as if the match was petering out to a draw, Defoe finished off a counter-attack led by fellow substitute James Milner before sending a 20-yard shot arrowing into Salvatore Sirigu's top-left corner.
The victory may not make up for the pain of June's Euro 2012 quarter-final defeat on penalties to the same opponents, but it was their first win against the Azzurri since a 2-0 friendly triumph in Nantes, France, in June 1997.
Just 52 days had passed since these nations met in Kiev, but the circumstances were entirely different in this game.
England and Italy took to the field with experimental sides, the focus firmly on the upcoming World Cup qualifying campaign.
Both coaches opted to hand chances to fringe players and young talent, with 19-year-old Jack Butland becoming England's youngest goalkeeper in 124 years.
He was rarely called upon in his 45 minutes on the field before he made way for Norwich City's John Ruddy, another novice.
But while a half-empty Wankdorf Stadium in Bern on Wednesday felt a million miles away from the tension of that night in Kiev on June 24, the pattern of the game was familiar early on.
Italy may have needed penalties to win that quarter-final, but Cesare Prandelli's side were far superior technically, and they appeared so again even without master creator Andrea Pirlo.
That said, the goal that gave them the lead after 15 minutes had something of an English stamp on it - after Butland had turned a deflected Alessandro Diamanti free-kick around the post, the former West Ham player swung in a corner that De Rossi headed into the roof of the net.
Proceedings livened up after that, with Adam Johnson forcing a good save from Sirigu before the debutant Mattia Destro shot over following a superb Alberto Aquilani pass over the top.
But then England equalised with a goal almost identical to that scored at the other end, with Jagielka heading in following a Frank Lampard right-wing corner.