It's D-Day for 'The Missile' with James Magnussen stepping out to the pool to compete for the first time since the disastrous 4 x 100m men's relay final.
Critics have launched into the reigning 100m freestyle world champion after his swim in the opening leg of the relay was almost a second outside his personal best.
He and relay teammate James Roberts, the fastest two men in the world this year in this event, will be out to prove the critics wrong by securing inside lanes for the semi-finals with good times in the heat.
Alicia Coutts and Stephanie Rice will be out to topple Chinese sensation Ye Shiwen in the 200m individual medley final after qualifying second and fifth fastest respectively.
The men's 200m freestyle relay team will be out to secure a medal after Australia won bronze in Beijing. The Americans will be favourites with the new-look Aussie team to be spearheaded by Kenrick Monk and Thomas Fraser-Holmes.
Rachel Bugg and four-time Olympian Loudy Wiggins will be in action as the medals are decided in the women's synchronised diving.
The Sharks will be back in the pool for their second match against Kazakhstan.
The Boomers will be underdogs in their second match against basketball powerhouse Spain.
There's also the second match of the Hockeyroos' campaign after their shock loss to New Zealand in the opening match.
The Australian men's volleyball team will go in as favourites against Great Britain in their second match.
There are two Aussies in action at ExCel Arena in the boxing, with Jeff Horn and Billy Ward in action in the light flyweight and the welterweight division respectively.
The eventing medals will be decided with the Australians out of contention after a disastrous day in the cross country.
It's a full day in the rowing at Eton Dorney with Australia's Olympic champions Scott Brennan and David Crawshay in action in the semi-finals of the men's double sculls.
Australia's lightweight men's four will follow Brennan and Crawshay.